Product: Anti-DLC1 & DLC2 mAb, clone 11F7
Cat. No.: MC-282 (100 μg)
Background:
The 10 kDa dynein light chain 1 (DLC1; LC8),
also known as neuronal nitric oxide synthase
inhibitory protein and PIN (protein inhibitor of
nNOS), is a component of cytoplasmic dynein,
which is involved in the minus end-directed
movement of organelles along microtubules. It is
a highly conserved protein that is associated
with the microtubular dynein motor complex by
its binding to dynein intermediate chain (DIC).
This allows DLC1 to act as a cargo protein,
hooking many different proteins to the dynein
motor complex. Binding of the BH3-only protein,
Bim, to DLC1 also controls its pro-apoptotic
activity. In healthy cells, most Bim molecules are
sequestered from Bcl-2 and its homologs by
DLC1 mediated binding to the dynein motor
complex. Certain apoptotic signals, such as
cytokine withdrawal or deregulated calcium flux,
induce the release of Bim and DLC1 from the
dynein motor complex. This allows Bim and
DLC1 to translocate and bind to Bcl-2 and its
homologs, thus neutralizing their anti-apoptotic
activity.
Specificity:
Recognizes human and mouse DLC1 and DLC2.
Species Reactivity:
Mouse and human. Others not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
Recombinant mouse DLC1 [LC8; PIN].
Format:
100 μg of 1 mg/mL liquid Protein G purified
monoclonal antibody in PBS containing 0.02%
sodium azide.
Storage:
Store at 4°C short term. For long term, store at
-20°C. Aliquot to avoid freeze/thaw cycles.
Applications:

Flow cytometry

Immunoprecipitation

Western blot
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.