GAPDH抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
GAPDH抗体

GAPDH抗体

商家询价

产品名称： GAPDH抗体

英文名称： GAPDHAntibody

产品编号： XY10494-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802 点击查看
  • 传真 : 点击查看
  • 邮箱 : shxysw02@163.com
  • 二维码 : 点击查看
进入展台

 GAPDH抗体

Applications
In-house applications ELISA, IHC, WB, IP, IF, FC     Application not tested? Please see our Guarantee.
Published applications IF, WB Publications 347 citations –view here
Species specificity Human,Mouse,Rat,Pig; other species not tested.
Published Species Bombyx, human, monkey, mouse, mussel, Oriental river prawns, pig, rat, Sea cucumbers, tree shrews, zebrafish
Images
Prev12NextView more validation data
  • 10494-1-AP;HeLa cellsWestern blot of Hela cell with anti-GAPDH (10494-1-AP) at various dilutions.
  • 10494-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 10494-1-AP(GAPDH antibody) at dilution of 1:5000
  • 10494-1-AP;A549 cellsIP Result of anti-GAPDH (IP:10494-1-AP, 3ug; Detection:10494-1-AP 1:3000) with A549 cells lysate 3500ug.
  • 10494-1-AP;human breast cancerImmunohistochemical of paraffin-embedded human breast cancer using 10494-1-AP(GAPDH antibody) at dilution of 1:100 (under 10x lens)
  • 10494-1-AP;HepG2 cellsImmunofluorescent analysis of HepG2 cells, using GAPDH antibody 10494-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 10494-1-AP;HEK-293 cells1X10^6 HEK-293 cells were stained with .2ug GAPDH antibody (10494-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IgG with dilution 1:100.
Positive WB detected in HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, human testis tissue, Jurkat cells, K-562 cells, MDA-MB-453s cells, mouse heart tissue, mouse kidney tissue, mouse lung tissue, PC-13 cells, Raji cells, rat brain tissue
Positive IP detected in A549 cells
Positive IHC detected in Human breast cancer tissue, human lung cancer tissue
Positive IF detected in HepG2 cells, Hela cells
Positive FC detected in HEK-293 cells
Recommended dilution WB : 1:2000-1:20000
IP : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:20-1:200
GAPDH抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen GAPDH fusion protein ag0766 Full name Glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular
weight		 36 kDa Observed molecular 
weight		 36 kDa
GenBank accession 
number		 BC004109 Gene ID (NCBI) 2597
Gene symbol GAPDH Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
BackgroundGAPDH抗体

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, RNA binding, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a rabbit polyclonal antibody raised against full-length GAPDH of human origin. It can recognize the 36kDa GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.GAPDH抗体

XY680Hu22 棕榈酰化膜蛋白2(MPP2)单克隆抗体 Monoclonal Antibody to Membrane Protein, Palmitoylated 2 (MPP2) Homo sapiens (Hukl)

XY681Hu22 乙醇脱氢酶1(ADH1)单克隆抗体 Monoclonal Antibody to Alcohol Dehydrogenase 1 (ADH1) Homo sapiens (Hukl)

XY683Hu22 Toll样受体6(TLR6)单克隆抗体 Monoclonal Antibody to Toll Like Receptor 6 (TLR6) Homo sapiens (Hukl)

XY684Hu22 粘蛋白5B(MUC5B)单克隆抗体 Monoclonal Antibody to Mucin 5 Subtype B (MUC5B) Homo sapiens (Hukl)

XY685Hu22 CD14分子(CD14)单克隆抗体 Monoclonal Antibody to Cluster Of Differentiation 14 (CD14) Homo sapiens (Hukl)

XY687Hu22 钙结合蛋白(CR)单克隆抗体 Monoclonal Antibody to Calretinin (CR) Homo sapiens (Hukl)

XY688Hu22 TGFβ诱导早期应答基因1(TIEG1)单克隆抗体 Monoclonal Antibody to TGF Beta Inducible Early Response Gene 1 (TIEG1) Homo sapiens (Hukl)

XY690Hu22 激肽释放酶8(KLK8)单克隆抗体 Monoclonal Antibody to Kallikrein 8 (KLK8) Homo sapiens (Hukl)

XY691Hu22 激肽释放酶6(KLK6)单克隆抗体 Monoclonal Antibody to Kallikrein 6 (KLK6) Homo sapiens (Hukl)

XY692Hu22 神经纤维网蛋白1(NRP1)单克隆抗体 Monoclonal Antibody to Neuropilin 1 (NRP1) Homo sapiens (Hukl)

XY693Hu22 27kDa热休克蛋白(HSPB1)单克隆抗体 Monoclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1) Homo sapiens (Hukl)

XY694Hu22 含血管性血友病因子A域蛋白3A(vWA3A)单克隆抗体 Monoclonal Antibody to Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) Homo sapiens (Hukl)

XY695Hu22 髓样前体细胞抑制因子2(MPIF2)单克隆抗体 Monoclonal Antibody to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) Homo sapiens (Hukl)

XY697Hu22 激肽释放酶10(KLK10)单克隆抗体 Monoclonal Antibody to Kallikrein 10 (KLK10) Homo sapiens (Hukl)

XY698Hu22 巨噬细胞移动抑制因子(MIF)单克隆抗体 Monoclonal Antibody to klrophage Migration Inhibitory Factor (MIF) Homo sapiens (Hukl)

XY699Hu22 环加氧酶2(PTGS2)单克隆抗体 Monoclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Homo sapiens (Hukl)

XY700Hu22 棕榈酰化膜蛋白6(MPP6)单克隆抗体 Monoclonal Antibody to Membrane Protein, Palmitoylated 6 (MPP6) Homo sapiens (Hukl)

XY702Hu22 硫氧化还原蛋白(Trx)单克隆抗体 Monoclonal Antibody to Thioredoxin (Trx) Homo sapiens (Hukl)

XY703Hu22 硫氧还蛋白还原酶1(TrxR1)单克隆抗体 Monoclonal Antibody to Thioredoxin Reductase 1 (TrxR1) Homo sapiens (Hukl)

XY704Hu22 载脂蛋白E(APOE)单克隆抗体 Monoclonal Antibody to Apolipoprotein E (APOE) Homo sapiens (Hukl)

XY705Hu22 粘蛋白2(MUC2)单克隆抗体 Monoclonal Antibody to Mucin 2 (MUC2) Homo sapiens (Hukl)

XY706Hu22 内脏脂肪特异性丝氨酸蛋白酶抑制因子(Vaspin)单克隆抗体 Monoclonal Antibody to Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) Homo sapiens (Hukl)

XY708Hu22 δ-促睡眠肽(δSIP)单克隆抗体 Monoclonal Antibody to Delta-Sleep Inducing Peptide (dSIP) Homo sapiens (Hukl)

XY709Hu22 Toll样受体9(TLR9)单克隆抗体 Monoclonal Antibody to Toll Like Receptor 9 (TLR9) Homo sapiens (Hukl)